XtalSpeed™ is a pre-packed chromatography separation column for protein used for crystallography.

ION-EXCHANGE RESINS[Open in a new window]

Characteristics

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XtalSpeed™ is a pre-packed chromatography column which is particularly effective for protein separation. The separated protein is use for crystallography or 3D conformation determination.

XtalSpeed™ has the following feature.

  1. 1.Working capability on medium pressure HPLC
  2. 2.Least non-specific protein binding
  3. 3.Column with various loading capacity
  4. 4.Durable for large sample loadings, preparative columns

Applications

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XtalSpeed™ is used for effective protein separation.

Lysozyme Separation

Condition

Column:
XtalSpeed™ SP01
Φ4.6×50mm
Eluent:
SolnA:50mM HEPES-NaOH(pH7.5)
SolnB:SolnA+1M NaCl
10→40%B/45min
Flow rate:
0.6mL/min

Purification of MW100kDa Protein

<Purification of basic kinase (MW c.a100kDa)>

Column Size

DA01:
Φ4.6×50mm
Competitor(S):
Φ4.6×100mm
Competitor(R):
Φ6.4×30mm
Load:
50µg
Eluent:
SolnA:50mM HEPES-NaOH(pH7)
SolnB:(SolnA)+1M NaCl
Gradient:0-40%B/40CV

Column List

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